WebbpLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17448 ; http://n2t.net/addgene:17448 ; RRID:Addgene_17448) For your … WebbUSP39 (NM06590.4) was inserted into pLenti-EF1a-FH-CMV-RFP-P2A-Puro vector (Vigene Inc.). The U87 cells with stable overexpressing USP39 were screened by RFP and …

Analysis of differentially expressed genes (DEGs) and miRNAs in …

Webbplent‑eF1a‑FH‑cMV‑GP were used to transfected cultured cells (5x105) using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in DMEM with 10% FBS at a multi‑ plicity of infection of 10 for 20 min. The lentiviral vector with green fluorescent protein (GFP) and resistance tag Webb27 dec. 2024 · Briefly, neuronal cells were infected with MAD2B-overexpressing lentivirus (LV-MAD2B-OE-eGFP) or control lentivirus (LV-eGFP; vector, pLent-EF1a-FH-CMV-GFP-P2A; titer, 1 × 10 8 U/mL) and cultured for another 3 days; neurons with or without lentiviral transduction were treated with CORT (100 μm) for 24 hours for subsequent experiments. life church streaming

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WebbPlvx Ef1a Ires Puro Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … Webb5 nov. 2024 · Context and Significance. Adipose dysfunction is a major contributor to obesity-associated atherosclerosis. In this study, Changtao Jiang and his colleagues find … Webb14 dec. 2024 · Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in … mcn heart ggc