WebThermo Fisher Scientific WebSeQuant ® HILIC (hydrophilic interaction liquid chromatography) HPLC columns constitute a range of high-performance tools for separating polar hydrophilic compounds. All columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1.
Understanding the HILIC Separation Method in LC - Restek
WebApr 2, 2024 · HILIC and RPLC are complementary separation methods . HILIC is separating lipids based on their headgroup polarity leading to coelution of all lipid molecular species of a corresponding subclass and its ISTD. RPLC is separating lipids mainly based on hydrophobicity of their fatty acid chains leading to separation of lipid molecular species … WebFigure 1. A robust and high performance HILIC LC/Q-TOF MS method was developed to improve the analytical selectivity, reproducibility, and coverage of metabolite for metabolome profiling using (A) 1290 Infinity Binary LC system coupled with 6545 Q-TOF (B) a newly developed HILIC column (p/n 673775-924) coupled with (C) a novel mobile phase … cindy coulter
Separation of ten phosphorylated mono-and disaccharides using HILIC …
WebThe sample solvent can have a big impact on peak shape in both reversed-phase and HILIC separations, especially when large volumes are injected. Diluting the sample with weak solvent can be an effect WebJun 15, 2024 · A high pressure HILIC-MS method was also reported for the rapid separation of lactose-1-phosphate (L1P), sucrose-6-phosphate (S6P) and trehalose-6-phosphate (T6P) within three minutes [15]. Nevertheless, a 30 min separation was used to obtain a better resolution between L1P, S6P and T6P and quantify T6P in Arabidopsis thaliana tissue [15] . WebThe separation achieved was similar on the 2 columns. However, the epinephrine/norepinephrine pair were slightly better separated on the Poroshell 120 HILIC column. Overall, the peaks were sharper on the Poroshell 120 HILIC column, and the last peak eluted at 3 minutes versus more than 6 minutes on the ZORBAX Rx-Sil column. … diabetes offerings